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13-week human fetal brain matchmaker cdna library  (Promega)

 
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    Structured Review

    Promega 13-week human fetal brain matchmaker cdna library
    13 Week Human Fetal Brain Matchmaker Cdna Library, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13-week+human+fetal+brain+matchmaker+cdna+library/13+week+human+fetal+brain+matchmaker+cdna+library/pm25546086-65-11-26
    Average 90 stars, based on 1 article reviews
    13-week human fetal brain matchmaker cdna library - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Y2H Assay:

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE).
    Article Snippet: Abbreviations BMP Bone morphogenetic protein BMPR BMP receptor DaPI 4,6-Diamidino-2-phenylindole DMeM Dulbecco’s modified eagle’s medium eCL enhanced chemiluminescence FBP F-box protein FBS Fetal bovine serum FBXO7 F-box only protein 7 heK293 human embryonic kidney 293 MaGe Melanoma-associated antigens NRaGe Neurotrophin receptor-interacting MaGe protein P19 P19 embryonic carcinoma PBS Phosphate-buffered saline PD Parkinson’s disease PI31 Proteasome inhibitor subunit 1 SCF Skp1–cullin1–F box protein Ubl Ubiquitin-like

    Article Title: Ubiquitin-specific protease 22 (USP22) positively regulates RCAN1 protein levels through RCAN1 de-ubiquitination.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved Protein ubiquitination can be reversed by de-ubiquitinating enzymes (DUBs), which are classified into two main classes, cysteine proteases and metalloproteases.. Cysteine proteases include ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases.

    cDNA Library Assay:

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE).
    Article Snippet: Abbreviations BMP Bone morphogenetic protein BMPR BMP receptor DaPI 4,6-Diamidino-2-phenylindole DMeM Dulbecco’s modified eagle’s medium eCL enhanced chemiluminescence FBP F-box protein FBS Fetal bovine serum FBXO7 F-box only protein 7 heK293 human embryonic kidney 293 MaGe Melanoma-associated antigens NRaGe Neurotrophin receptor-interacting MaGe protein P19 P19 embryonic carcinoma PBS Phosphate-buffered saline PD Parkinson’s disease PI31 Proteasome inhibitor subunit 1 SCF Skp1–cullin1–F box protein Ubl Ubiquitin-like

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE)
    Article Snippet: A control siRNA (NT-siRNA) with no known mammalian homology (siCONTROL non-targeting siRNA #1, Bioneer) was used as a negative control. siRNA duplexes were transfected into cells using Lipofectamine 2000, according to the manufacturer’s protocol. .. Screening was performed using a 13-week human fetal brain Matchmaker cDNA library sub-cloned into the activation domain (AD) in pACT2 vector (Promega) and a yeast L40 strain carrying the pHybLex/Zeo-plasmid encoding full-length wild-type FBXO7 isoform-2 as bait. ..

    Article Title: Ubiquitin-specific protease 22 (USP22) positively regulates RCAN1 protein levels through RCAN1 de-ubiquitination.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved Protein ubiquitination can be reversed by de-ubiquitinating enzymes (DUBs), which are classified into two main classes, cysteine proteases and metalloproteases.. Cysteine proteases include ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases.

    Activation Assay:

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE).
    Article Snippet: Abbreviations BMP Bone morphogenetic protein BMPR BMP receptor DaPI 4,6-Diamidino-2-phenylindole DMeM Dulbecco’s modified eagle’s medium eCL enhanced chemiluminescence FBP F-box protein FBS Fetal bovine serum FBXO7 F-box only protein 7 heK293 human embryonic kidney 293 MaGe Melanoma-associated antigens NRaGe Neurotrophin receptor-interacting MaGe protein P19 P19 embryonic carcinoma PBS Phosphate-buffered saline PD Parkinson’s disease PI31 Proteasome inhibitor subunit 1 SCF Skp1–cullin1–F box protein Ubl Ubiquitin-like

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE)
    Article Snippet: A control siRNA (NT-siRNA) with no known mammalian homology (siCONTROL non-targeting siRNA #1, Bioneer) was used as a negative control. siRNA duplexes were transfected into cells using Lipofectamine 2000, according to the manufacturer’s protocol. .. Screening was performed using a 13-week human fetal brain Matchmaker cDNA library sub-cloned into the activation domain (AD) in pACT2 vector (Promega) and a yeast L40 strain carrying the pHybLex/Zeo-plasmid encoding full-length wild-type FBXO7 isoform-2 as bait. ..

    Article Title: Ubiquitin-specific protease 22 (USP22) positively regulates RCAN1 protein levels through RCAN1 de-ubiquitination.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved Protein ubiquitination can be reversed by de-ubiquitinating enzymes (DUBs), which are classified into two main classes, cysteine proteases and metalloproteases.. Cysteine proteases include ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases.

    Plasmid Preparation:

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE).
    Article Snippet: Abbreviations BMP Bone morphogenetic protein BMPR BMP receptor DaPI 4,6-Diamidino-2-phenylindole DMeM Dulbecco’s modified eagle’s medium eCL enhanced chemiluminescence FBP F-box protein FBS Fetal bovine serum FBXO7 F-box only protein 7 heK293 human embryonic kidney 293 MaGe Melanoma-associated antigens NRaGe Neurotrophin receptor-interacting MaGe protein P19 P19 embryonic carcinoma PBS Phosphate-buffered saline PD Parkinson’s disease PI31 Proteasome inhibitor subunit 1 SCF Skp1–cullin1–F box protein Ubl Ubiquitin-like

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE)
    Article Snippet: A control siRNA (NT-siRNA) with no known mammalian homology (siCONTROL non-targeting siRNA #1, Bioneer) was used as a negative control. siRNA duplexes were transfected into cells using Lipofectamine 2000, according to the manufacturer’s protocol. .. Screening was performed using a 13-week human fetal brain Matchmaker cDNA library sub-cloned into the activation domain (AD) in pACT2 vector (Promega) and a yeast L40 strain carrying the pHybLex/Zeo-plasmid encoding full-length wild-type FBXO7 isoform-2 as bait. ..

    Article Title: Ubiquitin-specific protease 22 (USP22) positively regulates RCAN1 protein levels through RCAN1 de-ubiquitination.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved Protein ubiquitination can be reversed by de-ubiquitinating enzymes (DUBs), which are classified into two main classes, cysteine proteases and metalloproteases.. Cysteine proteases include ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases.

    Two Hybrid Screening:

    Article Title: The F-box protein FBXO7 positively regulates bone morphogenetic protein-mediated signaling through Lys-63-specific ubiquitination of neurotrophin receptor-interacting MAGE (NRAGE).
    Article Snippet: Abbreviations BMP Bone morphogenetic protein BMPR BMP receptor DaPI 4,6-Diamidino-2-phenylindole DMeM Dulbecco’s modified eagle’s medium eCL enhanced chemiluminescence FBP F-box protein FBS Fetal bovine serum FBXO7 F-box only protein 7 heK293 human embryonic kidney 293 MaGe Melanoma-associated antigens NRaGe Neurotrophin receptor-interacting MaGe protein P19 P19 embryonic carcinoma PBS Phosphate-buffered saline PD Parkinson’s disease PI31 Proteasome inhibitor subunit 1 SCF Skp1–cullin1–F box protein Ubl Ubiquitin-like



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    Promega 13-week human fetal brain matchmaker cdna library
    13 Week Human Fetal Brain Matchmaker Cdna Library, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13-week+human+fetal+brain+matchmaker+cdna+library/13+week+human+fetal+brain+matchmaker+cdna+library/pm25546086-65-11-26
    Average 90 stars, based on 1 article reviews
    13-week human fetal brain matchmaker cdna library - by Bioz Stars, 2026-09
    90/100 stars
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